› 3ngm

X-ray diffraction
2.8Å resolution

Crystal structure of lipase from Gibberella zeae

Released:
DOI: 10.2210/pdb3ngm/pdb
PDB entry 3ngm coloured by chain, front view.
PDB entry 3ngm coloured by chain, side view.
PDB entry 3ngm coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Crystal structure of a secreted lipase from Gibberella zeae reveals a novel "double-lock" mechanism.
Lou Z, Li M, Sun Y, Liu Y, Liu Z, Wu W, Rao Z
Protein Cell 1 760-70 (2010)
PMID: 21203917

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular lipase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 33.8 KDa
Source organism: Fusarium graminearum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q6WER3 (Residues: 31-349; Coverage: 95%)
Gene name: FGL1
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 78.417Å b: 91.001Å c: 195.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.264
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

New tools for exploring "old friends-microbial lipases".
Nagarajan S. (2012)
4 other articles

6 mentions without citation

Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries.
De Santi et al. (2016)
5 more