› 3ni7

X-ray diffraction
2.78Å resolution

Crystal structure of the TetR transcriptional regulator from Nitrosomonas europaea ATCC 19718

Released:
DOI: 10.2210/pdb3ni7/pdb
PDB entry 3ni7 coloured by chain, front view.
PDB entry 3ni7 coloured by chain, side view.
PDB entry 3ni7 coloured by chain, top view.
Source organism: Nitrosomonas europaea
Entry authors: Knapik A, Chruszcz M, Cymborowski M, Xu X, Savchenko A, Edwards A, Joachimiak A, Minor W, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 25.6 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82XH4 (Residues: 1-213; Coverage: 100%)
Gene name: NE0300
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 64.222Å b: 75.378Å c: 98.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.249 0.279
Expression system: Escherichia coli BL21(DE3)

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