› 3nia

X-ray diffraction
1.65Å resolution

GES-2 carbapenemase tazobactam complex

Released:
DOI: 10.2210/pdb3nia/pdb
PDB entry 3nia coloured by chain, front view.
PDB entry 3nia coloured by chain, side view.
PDB entry 3nia coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Frase H, Smith CA, Toth M, Vakulenko SB

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase GES-2 Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 29.31 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93F76 (Residues: 19-287; Coverage: 100%)
Gene names: bla, blaGES-2
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand TBE 1 x TBE
Ligand TF7 1 x TF7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P43212
Unit cell:
a: 83.44Å b: 83.44Å c: 70.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.193
Expression system: Escherichia coli

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