› 3njh

X-ray diffraction
1.94Å resolution

D37A mutant of SO1698 protein, an aspartic peptidase from Shewanella oneidensis.

Released:
DOI: 10.2210/pdb3njh/pdb
PDB entry 3njh coloured by chain, front view.
PDB entry 3njh coloured by chain, side view.
PDB entry 3njh coloured by chain, top view.
Source organism: Shewanella oneidensis
Primary publication:
Characterization of member of DUF1888 protein family, self-cleaving and self-assembling endopeptidase.
Osipiuk J, Mulligan R, Bargassa M, Hamilton JE, Cunningham MA, Joachimiak A
J Biol Chem 287 19452-61 (2012)
PMID: 22493430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-184384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autocatalytic aspartic peptidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.68 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8EGA7 (Residues: 1-125; Coverage: 100%)
Gene name: SO_1698
Sequence domains: DP-EP family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand NA 2 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3
Unit cell:
a: 97.986Å b: 97.986Å c: 65.844Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.127 0.152
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Efficient expression of nattokinase in Bacillus licheniformis: host strain construction and signal peptide optimization.
Wei et al. (2015)
1 more

1 mention without citation

Characterization of member of DUF1888 protein family, self-cleaving and self-assembling endopeptidase.
Osipiuk et al. (2012)