› 3njo

X-ray diffraction
2.47Å resolution

X-ray crystal structure of the Pyr1-pyrabactin A complex

Released:
DOI: 10.2210/pdb3njo/pdb
PDB entry 3njo coloured by chain, front view.
PDB entry 3njo coloured by chain, side view.
PDB entry 3njo coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for selective activation of ABA receptors.
Peterson FC, Burgie ES, Park SY, Jensen DR, Weiner JJ, Bingman CA, Chang CE, Cutler SR, Phillips GN, Volkman BF
Nat Struct Mol Biol 17 1109-13 (2010)
PMID: 20729860

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Abscisic acid receptor PYR1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 194 amino acids
Theoretical weight: 21.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O49686 (Residues: 1-191; Coverage: 100%)
Gene names: ABIP6, At4g17870, PYR1, RCAR11, T6K21.50
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

5 bound ligands:
Ligand PYV 2 x PYV
Ligand SO4 2 x SO4
Ligand NA 1 x NA
Ligand P2M 1 x P2M
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P6122
Unit cell:
a: 60.307Å b: 60.307Å c: 527.599Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.222 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Ion channels in plants.
Hedrich R. (2012)
11 more

5 mentions without citation

Chemical Manipulation of Abscisic Acid Signaling: A New Approach to Abiotic and Biotic Stress Management in Agriculture.
Hewage et al. (2020)
4 more