› 3njt

X-ray diffraction
3.5Å resolution

Crystal structure of the R450A mutant of the membrane protein FhaC

Released:
DOI: 10.2210/pdb3njt/pdb
PDB entry 3njt coloured by chain, front view.
PDB entry 3njt coloured by chain, side view.
PDB entry 3njt coloured by chain, top view.
Source organism: Bordetella pertussis
Primary publication:
Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily.
Delattre AS, Clantin B, Saint N, Locht C, Villeret V, Jacob-Dubuisson F
FEBS J 277 4755-65 (2010)
PMID: 20955520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152789 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamentous hemagglutinin transporter protein FhaC Chain: A
Molecule details ›
Chain: A
Length: 566 amino acids
Theoretical weight: 62.6 KDa
Source organism: Bordetella pertussis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35077 (Residues: 34-584; Coverage: 100%)
Gene names: BP1884, fhaC
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 107.55Å b: 139.39Å c: 113.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.35 0.35 0.38
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis.
Leyton et al. (2012)
8 more

4 mentions without citation

Contact-Dependent Growth Inhibition (CDI) and CdiB/CdiA Two-Partner Secretion Proteins.
Willett et al. (2015)
3 more