› 3nkc

X-ray diffraction
3.1Å resolution

Crystal structure of AqpZ F43W,H174G,T183F

Released:
DOI: 10.2210/pdb3nkc/pdb
Model geometry
Fit model/data
PDB entry 3nkc coloured by chain, front view.
PDB entry 3nkc coloured by chain, side view.
PDB entry 3nkc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural context shapes the aquaporin selectivity filter.
Savage DF, O'Connell JD, Miercke LJ, Finer-Moore J, Stroud RM
Proc Natl Acad Sci U S A 107 17164-9 (2010)
PMID: 20855585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aquaporin Z Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 24.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P60844 (Residues: 1-231; Coverage: 100%)
Gene names: JW0859, aqpZ, b0875, bniP
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

1 bound ligand:
Ligand BOG 3 x BOG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P4
Unit cell:
a: 92.892Å b: 92.892Å c: 79.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.222
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Diversity and evolution of membrane intrinsic proteins.
Abascal et al. (2014)
4 more

5 mentions without citation

Structural context shapes the aquaporin selectivity filter.
Savage et al. (2010)
4 more