› 3nmo

X-ray diffraction
3.1Å resolution

Crystal structure of an engineered monomeric CLC-ec1 Cl-/H+ transporter

Released:
DOI: 10.2210/pdb3nmo/pdb
PDB entry 3nmo coloured by chain, front view.
PDB entry 3nmo coloured by chain, side view.
PDB entry 3nmo coloured by chain, top view.
Source organism: Escherichia coli 042
Primary publication:
Design, function and structure of a monomeric ClC transporter.
Robertson JL, Kolmakova-Partensky L, Miller C
Nature 468 844-7 (2010)
PMID: 21048711

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
H(+)/Cl(-) exchange transporter ClcA Chain: A
Molecule details ›
Chain: A
Length: 465 amino acids
Theoretical weight: 49.8 KDa
Source organism: Escherichia coli 042
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 1-465; Coverage: 98%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 200.745Å b: 60.332Å c: 70.025Å
α: 90° β: 94.27° γ: 90°
R-values:
R R work R free
0.265 0.263 0.303
Expression system: Escherichia coli

Quick links

Citations

14 review citations

CLC Chloride Channels and Transporters: Structure, Function, Physiology, and Disease.
Jentsch et al. (2018)
13 more

3 mentions without citation

Design, function and structure of a monomeric ClC transporter.
Robertson et al. (2010)
2 more