› 3nmr

X-ray diffraction
1.85Å resolution

Crystal Structure of CUGBP1 RRM1/2-RNA Complex

Released:
DOI: 10.2210/pdb3nmr/pdb
PDB entry 3nmr coloured by chain, front view.
PDB entry 3nmr coloured by chain, side view.
PDB entry 3nmr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into RNA recognition by the alternate-splicing regulator CUG-binding protein 1.
Teplova M, Song J, Gaw HY, Teplov A, Patel DJ
Structure 18 1364-77 (2010)
PMID: 20947024

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187793 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CUGBP Elav-like family member 1 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92879 (Residues: 14-187; Coverage: 36%)
Gene names: BRUNOL2, CELF1, CUGBP, CUGBP1, NAB50
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*GP*UP*UP*GP*UP*UP*UP*UP*GP*UP*UP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I222
Unit cell:
a: 47.548Å b: 70.012Å c: 132.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

12 review citations

The importance of CELF control: molecular and biological roles of the CUG-BP, Elav-like family of RNA-binding proteins.
Dasgupta et al. (2012)
11 more

10 mentions without citation

mRNA localization in metazoans: A structural perspective.
Lazzaretti et al. (2017)
9 more