› 3nns

X-ray diffraction
1.9Å resolution

BeF3 Activated DrrB Receiver Domain

Released:
DOI: 10.2210/pdb3nns/pdb
PDB entry 3nns coloured by chain, front view.
PDB entry 3nns coloured by chain, side view.
PDB entry 3nns coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Regulation of response regulator autophosphorylation through interdomain contacts.
Barbieri CM, Mack TR, Robinson VL, Miller MT, Stock AM
J Biol Chem 285 32325-35 (2010)
PMID: 20702407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.79 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WXY0 (Residues: 1-117; Coverage: 53%)
Gene name: TM_0126
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand BEF 2 x BEF
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 64.32Å b: 37.492Å c: 91.478Å
α: 90° β: 108.15° γ: 90°
R-values:
R R work R free
0.189 0.189 0.231
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
5 more

3 mentions without citation

Regulation of response regulator autophosphorylation through interdomain contacts.
Barbieri et al. (2010)
2 more