› 3no4

X-ray diffraction
2Å resolution

Crystal structure of a creatinine amidohydrolase (Npun_F1913) from Nostoc punctiforme PCC 73102 at 2.00 A resolution

Released:
DOI: 10.2210/pdb3no4/pdb
PDB entry 3no4 coloured by chain, front view.
PDB entry 3no4 coloured by chain, side view.
PDB entry 3no4 coloured by chain, top view.
Source organism: Nostoc punctiforme PCC 73102
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Creatininase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 267 amino acids
Theoretical weight: 29.24 KDa
Source organism: Nostoc punctiforme PCC 73102
Expression system: Escherichia coli
UniProt:
  • Canonical: B2J3U5 (Residues: 1-248; Coverage: 100%)
Gene name: Npun_F1913
Sequence domains: Creatinine amidohydrolase
Structure domains: Creatininase

Ligands and Environments

4 bound ligands:
Ligand NI 3 x NI
Ligand UNL 3 x UNL
Ligand CL 1 x CL
Ligand EDO 16 x EDO
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 89.085Å b: 89.085Å c: 211.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.192
Expression system: Escherichia coli

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