› 3no5

X-ray diffraction
1.9Å resolution

Crystal structure of a Pfam DUF849 domain containing protein (Reut_A1631) from Ralstonia eutropha JMP134 at 1.90 A resolution

Released:
DOI: 10.2210/pdb3no5/pdb
PDB entry 3no5 coloured by chain, front view.
PDB entry 3no5 coloured by chain, side view.
PDB entry 3no5 coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175223 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-keto-5-aminohexanoate cleavage enzyme Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 275 amino acids
Theoretical weight: 30.14 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q471D6 (Residues: 1-274; Coverage: 100%)
Gene name: Reut_A1631
Sequence domains: beta-keto acid cleavage enzyme
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand EDO 10 x EDO
Ligand ACT 3 x ACT
1 modified residue:
Ligand MSE 66 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 83.181Å b: 81.221Å c: 140.429Å
α: 90° β: 103.68° γ: 90°
R-values:
R R work R free
0.207 0.206 0.224
Expression system: Escherichia coli

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