› 3no6

X-ray diffraction
1.65Å resolution

Crystal structure of a putative thiaminase II (SE1693) from Staphylococcus epidermidis ATCC 12228 at 1.65 A resolution

Released:
DOI: 10.2210/pdb3no6/pdb
PDB entry 3no6 coloured by chain, front view.
PDB entry 3no6 coloured by chain, side view.
PDB entry 3no6 coloured by chain, top view.
Source organism: Staphylococcus epidermidis ATCC 12228
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminopyrimidine aminohydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 29.81 KDa
Source organism: Staphylococcus epidermidis ATCC 12228
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8CNK1 (Residues: 1-229; Coverage: 100%)
Gene names: SE_1693, tenA
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

5 bound ligands:
Ligand IMD 4 x IMD
Ligand ACT 6 x ACT
Ligand MPD 7 x MPD
Ligand MRD 3 x MRD
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2, SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 70.155Å b: 92.713Å c: 165.037Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.19
Expression system: Escherichia coli

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