› 3no7

X-ray diffraction
1.4Å resolution

Crystal structure of the centromere-binding protein ParB from plasmid pCXC100

Released:
DOI: 10.2210/pdb3no7/pdb
PDB entry 3no7 coloured by chain, front view.
PDB entry 3no7 coloured by chain, side view.
PDB entry 3no7 coloured by chain, top view.
Source organism: Leifsonia xyli subsp. cynodontis
Primary publication:
Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.
Huang L, Yin P, Zhu X, Zhang Y, Ye K
Nucleic Acids Res 39 2954-68 (2011)
PMID: 21123191

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179576 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Centromere-binding protein ParB C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 80 amino acids
Theoretical weight: 9.02 KDa
Source organism: Leifsonia xyli subsp. cynodontis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6EEF9 (Residues: 65-139; Coverage: 54%)
Sequence domains: Centromere-binding protein ParB C-terminal
Structure domains: Arc Repressor Mutant

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 74.186Å b: 74.186Å c: 52.596Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.197 0.212
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Segrosome Complex Formation during DNA Trafficking in Bacterial Cell Division.
Oliva MA. (2016)
1 more

1 mention without citation

Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.
Huang et al. (2011)