› 3nr1

X-ray diffraction
1.9Å resolution

A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses

Released:
DOI: 10.2210/pdb3nr1/pdb
PDB entry 3nr1 coloured by chain, front view.
PDB entry 3nr1 coloured by chain, side view.
PDB entry 3nr1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses.
Sun D, Lee G, Lee JH, Kim HY, Rhee HW, Park SY, Kim KJ, Kim Y, Kim BY, Hong JI, Park C, Choy HE, Kim JH, Jeon YH, Chung J
Nat Struct Mol Biol 17 1188-94 (2010)
PMID: 20818390

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-bis(diphosphate) + H(2)O = guanosine 5'-diphosphate + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N4P3 (Residues: 2-179; Coverage: 99%)
Gene names: HDDC3, MESH1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A, PAL/PLS BEAMLINE 6C1
Spacegroup: P21
Unit cell:
a: 53.9Å b: 62.4Å c: 53.9Å
α: 90° β: 95.3° γ: 90°
R-values:
R R work R free
0.203 0.203 0.23
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Recent functional insights into the role of (p)ppGpp in bacterial physiology.
Hauryliuk et al. (2015)
24 more

5 mentions without citation

Metazoan stringent-like response mediated by MESH1 phenotypic conservation via distinct mechanisms.
Mestre et al. (2022)
4 more