› 3nrb

X-ray diffraction
2.05Å resolution

Crystal structure of a formyltetrahydrofolate deformylase (purU, PP_1943) from PSEUDOMONAS PUTIDA KT2440 at 2.05 A resolution

Released:
DOI: 10.2210/pdb3nrb/pdb
PDB entry 3nrb coloured by chain, front view.
PDB entry 3nrb coloured by chain, side view.
PDB entry 3nrb coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyltetrahydrofolate deformylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 287 amino acids
Theoretical weight: 32.4 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88LI9 (Residues: 1-286; Coverage: 100%)
Gene names: PP_1943, purU, purU-III
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NA 3 x NA
Ligand UNL 2 x UNL
Ligand FLC 2 x FLC
Ligand EDO 7 x EDO
Ligand IMD 4 x IMD
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 82.452Å b: 118.245Å c: 129.193Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.24
Expression system: Escherichia coli

Quick links