› 3nrp

X-ray diffraction
1.6Å resolution

Crystal structure of 'as isolated' uropathogenic E. coli strain F11 FetP recombinantly expressed in the periplasm of E. coli BL21(DE3)

Released:
DOI: 10.2210/pdb3nrp/pdb
PDB entry 3nrp coloured by chain, front view.
PDB entry 3nrp coloured by chain, side view.
PDB entry 3nrp coloured by chain, top view.
Source organism: Escherichia coli F11
Primary publication:
Characterization of a dipartite iron uptake system from uropathogenic Escherichia coli strain F11.
Koch D, Chan AC, Murphy ME, Lilie H, Grass G, Nies DH
J Biol Chem 286 25317-30 (2011)
PMID: 21596746

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic protein-probably involved in high-affinity Fe2+ transport Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 160 amino acids
Theoretical weight: 17.66 KDa
Source organism: Escherichia coli F11
Expression system: Escherichia coli BL21(DE3)
Structure domains: Periplasmic metal-binding protein Tp34-type

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P32
Unit cell:
a: 134.852Å b: 134.852Å c: 45.422Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Virulence and Fitness Determinants of Uropathogenic Escherichia coli.
Subashchandrabose et al. (2015)
5 more

1 mention without citation

Characterization of a dipartite iron uptake system from uropathogenic Escherichia coli strain F11.
Koch et al. (2011)