› 3nry

X-ray diffraction
2Å resolution

Insights into anti-parallel microtubule crosslinking by PRC1, a conserved microtubule binding protein

Released:
DOI: 10.2210/pdb3nry/pdb
PDB entry 3nry coloured by chain, front view.
PDB entry 3nry coloured by chain, side view.
PDB entry 3nry coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.
Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM
Cell 142 433-43 (2010)
PMID: 20691902

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein regulator of cytokinesis 1 Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 16.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43663 (Residues: 341-466; Coverage: 20%)
Gene name: PRC1
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P62
Unit cell:
a: 51.043Å b: 51.043Å c: 90.347Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.238 0.274
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Molecular control of animal cell cytokinesis.
Fededa et al. (2012)
26 more

2 mentions without citation

Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.
Subramanian et al. (2010)
1 more