› 3nsg

X-ray diffraction
2.79Å resolution

Crystal Structure of OmpF, an Outer Membrane Protein from Salmonella typhi

Released:
DOI: 10.2210/pdb3nsg/pdb
PDB entry 3nsg coloured by chain, front view.
PDB entry 3nsg coloured by chain, side view.
PDB entry 3nsg coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhi str. Ty21a
Primary publication:
Asymmetric pore occupancy in crystal structure of OmpF porin from Salmonella typhi.
Balasubramaniam D, Arockiasamy A, Kumar PD, Sharma A, Krishnaswamy S
J Struct Biol 178 233-44 (2012)
PMID: 22525817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 341 amino acids
Theoretical weight: 38.22 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhi str. Ty21a
Expression system: Escherichia coli
UniProt:
  • Canonical: P37432 (Residues: 24-363; Coverage: 100%)
Gene names: STM0999, ompF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

6 bound ligands:
Ligand SO4 80 x SO4
Ligand GOL 38 x GOL
Ligand LDA 17 x LDA
Ligand TAM 6 x TAM
Ligand FLC 4 x FLC
Ligand TLA 2 x TLA
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 85.185Å b: 139.335Å c: 150.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.255 0.337
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria.
Vergalli et al. (2020)
1 more

5 mentions without citation

Asymmetric pore occupancy in crystal structure of OmpF porin from Salmonella typhi.
Balasubramaniam et al. (2012)
4 more