› 3nsj

X-ray diffraction
2.75Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Perforin-1 Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 60.8 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10820 (Residues: 21-554; Coverage: 100%)
Gene names: Pfp, Prf1
Sequence domains:
Structure domains: C2 domain

Ligands and Environments

5 bound ligands:
Ligand GOL 3 x GOL
Ligand CA 2 x CA
Ligand IOD 4 x IOD
Ligand CL 2 x CL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 78.64Å b: 109.8Å c: 141.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.215
Expression system: Spodoptera frugiperda

Quick links

Citations

68 review citations

Perforin and granzymes: function, dysfunction and human pathology.
Voskoboinik et al. (2015)
67 more

19 mentions without citation

Membrane assembly of the cholesterol-dependent cytolysin pore complex.
Hotze et al. (2012)
18 more