› 3nsu

X-ray diffraction
2Å resolution

A Systematic Screen for Protein-Lipid Interactions in Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3nsu/pdb
PDB entry 3nsu coloured by chain, front view.
PDB entry 3nsu coloured by chain, side view.
PDB entry 3nsu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A systematic screen for protein-lipid interactions in Saccharomyces cerevisiae.
Gallego O, Betts MJ, Gvozdenovic-Jeremic J, Maeda K, Matetzki C, Aguilar-Gurrieri C, Beltran-Alvarez P, Bonn S, Fernández-Tornero C, Jensen LJ, Kuhn M, Trott J, Rybin V, Müller CW, Bork P, Kaksonen M, Russell RB, Gavin AC
Mol Syst Biol 6 430 (2010)
PMID: 21119626

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.78 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40485 (Residues: 469-583; Coverage: 17%)
Gene names: LIT2, SLM1, YIL105C
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 82.39Å b: 73.05Å c: 37.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

39 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
38 more

3 mentions without citation

Conditional peripheral membrane proteins: facing up to limited specificity.
Moravcevic et al. (2012)
2 more