› 3nt3

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE OF LSSmKate2 red fluorescent proteins with large Stokes shift

Released:
DOI: 10.2210/pdb3nt3/pdb
PDB entry 3nt3 coloured by chain, front view.
PDB entry 3nt3 coloured by chain, side view.
PDB entry 3nt3 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large Stokes shift.
Piatkevich KD, Malashkevich VN, Almo SC, Verkhusha VV
J Am Chem Soc 132 10762-70 (2010)
PMID: 20681709

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-163824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LSSmKate2 red fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 243 amino acids
Theoretical weight: 27.61 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand NRQ 4 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 71.704Å b: 49.454Å c: 127.171Å
α: 90° β: 93.13° γ: 90°
R-values:
R R work R free
0.175 0.174 0.202
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Fluorescent proteins for live-cell imaging with super-resolution.
Nienhaus et al. (2014)
8 more

4 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
3 more