› 3nt7

X-ray diffraction
2.4Å resolution

Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant

Released:
DOI: 10.2210/pdb3nt7/pdb
PDB entry 3nt7 coloured by chain, front view.
PDB entry 3nt7 coloured by chain, side view.
PDB entry 3nt7 coloured by chain, top view.
Source organism: Vaccinia virus Western Reserve
Primary publication:
Vaccinia virus D4 mutants defective in processive DNA synthesis retain binding to A20 and DNA.
Druck Shudofsky AM, Silverman JE, Chattopadhyay D, Ricciardi RP
J Virol 84 12325-35 (2010)
PMID: 20861259

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-137589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chains: A, C
Molecule details ›
Chains: A, C
Length: 238 amino acids
Theoretical weight: 27.2 KDa
Source organism: Vaccinia virus Western Reserve
Expression system: Escherichia coli
UniProt:
  • Canonical: P04303 (Residues: 1-218; Coverage: 100%)
Gene names: D4R, OPG116, UNG, VACWR109
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 85.195Å b: 85.195Å c: 139.439Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.222 0.274
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Uracil-DNA glycosylases-structural and functional perspectives on an essential family of DNA repair enzymes.
Schormann et al. (2014)
2 more

4 mentions without citation

Poxvirus uracil-DNA glycosylase-An unusual member of the family I uracil-DNA glycosylases.
Schormann et al. (2016)
3 more