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› 3nu7

X-ray diffraction
1.95Å resolution

WbpE, an Aminotransferase from Pseudomonas aeruginosa Involved in O-antigen Assembly in Complex with the Cofactor PMP

Released:
DOI: 10.2210/pdb3nu7/pdb
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PDB entry 3nu7 coloured by chain, front view.
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Source organism: Pseudomonas aeruginosa
Primary publication:
Structural analysis of WbpE from Pseudomonas aeruginosa PAO1: a nucleotide sugar aminotransferase involved in O-antigen assembly.
Larkin A, Olivier NB, Imperiali B
Biochemistry 49 7227-37 (2010)
PMID: 20604544

Function and Biology Details

Reaction catalysed: 
UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate = UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 38.97 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HZ76 (Residues: 1-359; Coverage: 100%)
Gene names: PA3155, wbpE
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 2 x PMP
1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21212
Unit cell:
a: 78.28Å b: 149.288Å c: 54.898Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.246
Expression system: Escherichia coli

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Citations

3 review citations

N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.
Jarrell et al. (2014)
2 more

2 mentions without citation

Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis.
Singh et al. (2015)
1 more

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