› 3nua

X-ray diffraction
1.4Å resolution

Crystal Structure of Phosphoribosylaminoimidazole-Succinocarboxamide Synthase from Clostridium perfringens

Released:
DOI: 10.2210/pdb3nua/pdb
PDB entry 3nua coloured by chain, front view.
PDB entry 3nua coloured by chain, side view.
PDB entry 3nua coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Entry authors: Kim Y, Mulligan R, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.31 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q0TTB4 (Residues: 1-235; Coverage: 100%)
Gene names: CPF_0673, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ADP 3 x ADP
Ligand AMP 1 x AMP
Ligand CIT 2 x CIT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 80.877Å b: 148.913Å c: 45.315Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.161
Expression system: Escherichia coli BL21

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