› 3nxj

X-ray diffraction
1.97Å resolution

Crystal Structure of Ketosteroid Isomerase D99N from Pseudomonas Testosteroni (tKSI)

Released:
DOI: 10.2210/pdb3nxj/pdb
Model geometry
Fit model/data
PDB entry 3nxj coloured by chain, front view.
PDB entry 3nxj coloured by chain, side view.
PDB entry 3nxj coloured by chain, top view.
Source organism: Comamonas testosteroni
Entry authors: Gonzalez A, Tsai Y, Schwans J, Sunden F, Herschlag D

Function and Biology Details

Reaction catalysed: 
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-133706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 13.41 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00947 (Residues: 1-125; Coverage: 100%)
Gene name: ksi
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2, SSRL BEAMLINE BL9-1
Spacegroup: C2221
Unit cell:
a: 60.357Å b: 104.786Å c: 141.435Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.252 0.314
Expression system: Escherichia coli BL21

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