› 3nyt

X-ray diffraction
1.3Å resolution

X-ray crystal structure of the WlbE (WpbE) aminotransferase from pseudomonas aeruginosa, mutation K185A, in complex with the PLP external aldimine adduct with UDP-3-amino-2-N-acetyl-glucuronic acid, at 1.3 angstrom resolution

Released:
DOI: 10.2210/pdb3nyt/pdb
PDB entry 3nyt coloured by chain, front view.
PDB entry 3nyt coloured by chain, side view.
PDB entry 3nyt coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural investigation on WlaRG from Campylobacter jejuni: A sugar aminotransferase.
Dow GT, Gilbert M, Thoden JB, Holden HM
Protein Sci 26 586-599 (2017)
PMID: 28028852

Function and Biology Details

Reaction catalysed: 
UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate = UDP-2-acetamido-2-deoxy-D-ribo-hex-3-uluronate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 39.98 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HZ76 (Residues: 1-359; Coverage: 100%)
Gene names: PA3155, wbpE
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ULP 1 x ULP
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 59.684Å b: 92.17Å c: 75.652Å
α: 90° β: 111.84° γ: 90°
R-values:
R R work R free
0.191 0.19 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Investigation of a sugar N-formyltransferase from the plant pathogen Pantoea ananatis.
Hofmeister et al. (2019)
5 more

2 mentions without citation

Structure of the external aldimine form of PglE, an aminotransferase required for N,N'-diacetylbacillosamine biosynthesis.
Riegert et al. (2015)
1 more