› 3o0e

X-ray diffraction
3.01Å resolution

Crystal structure of OmpF in complex with colicin peptide OBS1

Released:
DOI: 10.2210/pdb3o0e/pdb
PDB entry 3o0e coloured by chain, front view.
PDB entry 3o0e coloured by chain, side view.
PDB entry 3o0e coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF.
Housden NG, Wojdyla JA, Korczynska J, Grishkovskaya I, Kirkpatrick N, Brzozowski AM, Kleanthous C
Proc Natl Acad Sci U S A 107 21412-7 (2010)
PMID: 21098297

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136402 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer membrane porin F Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 340 amino acids
Theoretical weight: 37.11 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin
Colicin-E9 Chains: L, M, N, O, P, Q
Molecule details ›
Chains: L, M, N, O, P, Q
Length: 17 amino acids
Theoretical weight: 1.56 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P09883 (Residues: 2-18; Coverage: 3%)
Gene names: cei, col

Ligands and Environments

1 bound ligand:
Ligand BOG 6 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 101.491Å b: 101.618Å c: 162.142Å
α: 90° β: 94.46° γ: 90°
R-values:
R R work R free
0.267 0.265 0.302
Expression system: Not provided

Quick links

Citations

14 review citations

Twenty years of gas phase structural biology.
Marcoux et al. (2013)
13 more

7 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
6 more