› 3o10

X-ray diffraction
1.9Å resolution

Crystal structure of the HEPN domain from human sacsin

Released:
DOI: 10.2210/pdb3o10/pdb
PDB entry 3o10 coloured by chain, front view.
PDB entry 3o10 coloured by chain, side view.
PDB entry 3o10 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of defects in the sacsin HEPN domain responsible for autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS).
Kozlov G, Denisov AY, Girard M, Dicaire MJ, Hamlin J, McPherson PS, Brais B, Gehring K
J Biol Chem 286 20407-12 (2011)
PMID: 21507954

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sacsin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 141 amino acids
Theoretical weight: 16.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NZJ4 (Residues: 4440-4579; Coverage: 3%)
Gene names: DNAJC29, KIAA0730, SACS
Sequence domains: HEPN domain
Structure domains: Nucleotidyltransferases domain 2

Ligands and Environments

1 bound ligand:
Ligand MLI 3 x MLI
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3221
Unit cell:
a: 72.581Å b: 72.581Å c: 201.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Molecular chaperones and neuronal proteostasis.
Smith et al. (2015)
4 more

5 mentions without citation

Structural basis of defects in the sacsin HEPN domain responsible for autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS).
Kozlov et al. (2011)
4 more