› 3o4u

X-ray diffraction
2.25Å resolution

Crystal Structure of HePTP with an Atypically Open WPD Loop

Released:
DOI: 10.2210/pdb3o4u/pdb
PDB entry 3o4u coloured by chain, front view.
PDB entry 3o4u coloured by chain, side view.
PDB entry 3o4u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Visualizing active-site dynamics in single crystals of HePTP: opening of the WPD loop involves coordinated movement of the E loop.
Critton DA, Tautz L, Page R
J Mol Biol 405 619-29 (2011)
PMID: 21094165

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 7 Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 35.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35236 (Residues: 65-360; Coverage: 82%)
Gene name: PTPN7
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

3 bound ligands:
Ligand TLA 1 x TLA
Ligand SRT 1 x SRT
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 119.035Å b: 39.207Å c: 83.981Å
α: 90° β: 124.23° γ: 90°
R-values:
R R work R free
0.193 0.19 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Protein Tyrosine Phosphatases: A new paradigm in an old signaling system?
Welsh et al. (2021)
1 more

3 mentions without citation

Protein tyrosine phosphatases: structure, function, and implication in human disease.
Tautz et al. (2013)
2 more