› 3o7o

X-ray diffraction
2.41Å resolution

Use of synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima

Released:
DOI: 10.2210/pdb3o7o/pdb
PDB entry 3o7o coloured by chain, front view.
PDB entry 3o7o coloured by chain, side view.
PDB entry 3o7o coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima.
Forse GJ, Ram N, Banatao DR, Cascio D, Sawaya MR, Klock HE, Lesley SA, Yeates TO
Protein Sci 20 168-78 (2011)
PMID: 21082721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.68 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9S5X8 (Residues: 1-258; Coverage: 100%)
Gene name: TM_1524
Sequence domains: Glycosyl hydrolase family 12
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand BR 7 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, APS BEAMLINE 24-ID-E
Spacegroup: P63
Unit cell:
a: 114.028Å b: 114.028Å c: 98.818Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.176 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
7 other articles

2 mentions without citation

Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design.
Zhang et al. (2015)
1 more