› 3o7q

X-ray diffraction
3.14Å resolution

Crystal structure of a Major Facilitator Superfamily (MFS) transporter, FucP, in the outward conformation

Released:
DOI: 10.2210/pdb3o7q/pdb
PDB entry 3o7q coloured by chain, front view.
PDB entry 3o7q coloured by chain, side view.
PDB entry 3o7q coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of a fucose transporter in an outward-open conformation.
Dang S, Sun L, Huang Y, Lu F, Liu Y, Gong H, Wang J, Yan N
Nature 467 734-8 (2010)
PMID: 20877283

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-fucose-proton symporter Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 47.57 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11551 (Residues: 1-438; Coverage: 100%)
Gene names: JW2772, b2801, fucP
Sequence domains: Sugar (and other) transporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

1 bound ligand:
Ligand BNG 1 x BNG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3221
Unit cell:
a: 119.46Å b: 119.46Å c: 98.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

33 review citations

The SLC2 (GLUT) family of membrane transporters.
Mueckler et al. (2013)
32 more

42 mentions without citation

Energy coupling mechanisms of MFS transporters.
Zhang et al. (2015)
41 more