› 3o8q

X-ray diffraction
1.45Å resolution

1.45 Angstrom Resolution Crystal Structure of Shikimate 5-Dehydrogenase (aroE) from Vibrio cholerae

Released:
DOI: 10.2210/pdb3o8q/pdb
PDB entry 3o8q coloured by chain, front view.
PDB entry 3o8q coloured by chain, side view.
PDB entry 3o8q coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Minasov G, Light SH, Shuvalova L, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163852 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Shikimate 5-dehydrogenase I alpha Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 30.55 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
Structure domains:
Shikimate 5-dehydrogenase I alpha Chain: B
Molecule details ›
Chain: B
Length: 275 amino acids
Theoretical weight: 29.97 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand SO4 4 x SO4
Ligand EPE 1 x EPE
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 56.871Å b: 63.146Å c: 136.842Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.195
Expression system: Escherichia coli

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