› 3ob4

X-ray diffraction
2.71Å resolution

MBP-fusion protein of the major peanut allergen Ara h 2

Released:
DOI: 10.2210/pdb3ob4/pdb
PDB entry 3ob4 coloured by chain, front view.
PDB entry 3ob4 coloured by chain, side view.
PDB entry 3ob4 coloured by chain, top view.
Source organisms:
Primary publication:
Ara h 2: crystal structure and IgE binding distinguish two subpopulations of peanut allergic patients by epitope diversity.
Mueller GA, Gosavi RA, Pomés A, Wünschmann S, Moon AF, London RE, Pedersen LC
Allergy 66 878-85 (2011)
PMID: 21255036

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107799 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 55.66 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: A5Z1Q8 (Residues: 31-160; Coverage: 94%)
Gene names: Ara d 2.01, Ara h 2, JW3994, LOC107462439, ara d 2, b4034, conglutin, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 3 x SO4
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 68.847Å b: 87.381Å c: 113.137Å
α: 90° β: 103.93° γ: 90°
R-values:
R R work R free
0.187 0.183 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Common food allergens and their IgE-binding epitopes.
Matsuo et al. (2015)
5 more

22 mentions without citation

Peanut allergens.
Palladino et al. (2018)
21 more