› 3ob6

X-ray diffraction
3Å resolution

Structure of AdiC(N101A) in the open-to-out Arg+ bound conformation

Released:
DOI: 10.2210/pdb3ob6/pdb
PDB entry 3ob6 coloured by chain, front view.
PDB entry 3ob6 coloured by chain, side view.
PDB entry 3ob6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular basis of substrate-induced permeation by an amino acid antiporter.
Kowalczyk L, Ratera M, Paladino A, Bartoccioni P, Errasti-Murugarren E, Valencia E, Portella G, Bial S, Zorzano A, Fita I, Orozco M, Carpena X, Vázquez-Ibar JL, Palacín M
Proc Natl Acad Sci U S A 108 3935-40 (2011)
PMID: 21368142

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157970 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine/agmatine antiporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 46.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60061 (Residues: 1-445; Coverage: 100%)
Gene names: JW4076, adiC, aniC, b4115, yjdD, yjdE
Sequence domains: Amino acid permease

Ligands and Environments

1 bound ligand:
Ligand ARG 2 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 86.071Å b: 77.204Å c: 104.451Å
α: 90° β: 106.79° γ: 90°
R-values:
R R work R free
0.245 0.243 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

The SLC3 and SLC7 families of amino acid transporters.
Fotiadis et al. (2013)
15 more

20 mentions without citation

Membrane transporters for the special amino acid glutamine: structure/function relationships and relevance to human health.
Pochini et al. (2014)
19 more