› 3of5

X-ray diffraction
1.52Å resolution

Crystal Structure of a Dethiobiotin Synthetase from Francisella tularensis subsp. tularensis SCHU S4

Released:
DOI: 10.2210/pdb3of5/pdb
PDB entry 3of5 coloured by chain, front view.
PDB entry 3of5 coloured by chain, side view.
PDB entry 3of5 coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Brunzelle JS, Skarina T, Gordon E, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent dethiobiotin synthetase BioD Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.54 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NGB5 (Residues: 1-225; Coverage: 100%)
Gene names: FTT_0934c, bioD
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ACT 3 x ACT
Ligand NA 5 x NA
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 39.278Å b: 93.177Å c: 62.732Å
α: 90° β: 95.78° γ: 90°
R-values:
R R work R free
0.171 0.169 0.198
Expression system: Escherichia coli

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