› 3ofj

X-ray diffraction
2.43Å resolution

Crystal structure of N-methyltransferase NodS from Bradyrhizobium japonicum WM9

Released:
DOI: 10.2210/pdb3ofj/pdb
PDB entry 3ofj coloured by chain, front view.
PDB entry 3ofj coloured by chain, side view.
PDB entry 3ofj coloured by chain, top view.
Source organism: Bradyrhizobium sp. WM9
Primary publication:
Crystal structures of NodS N-methyltransferase from Bradyrhizobium japonicum in ligand-free form and as SAH complex.
Cakici O, Sikorski M, Stepkowski T, Bujacz G, Jaskolski M
J Mol Biol 404 874-89 (2010)
PMID: 20970431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nodulation protein S Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.15 KDa
Source organism: Bradyrhizobium sp. WM9
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AQ22 (Residues: 1-209; Coverage: 100%)
Gene name: nodS
Sequence domains: Nodulation protein S (NodS)
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P4322
Unit cell:
a: 48.7Å b: 48.7Å c: 141.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.203 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
1 more