› 3ohg

X-ray diffraction
1.8Å resolution

Crystal structure of a protein with unknown function from DUF2233 family (BACOVA_00430) from Bacteroides ovatus at 1.80 A resolution

Released:
DOI: 10.2210/pdb3ohg/pdb
PDB entry 3ohg coloured by chain, front view.
PDB entry 3ohg coloured by chain, side view.
PDB entry 3ohg coloured by chain, top view.
Source organism: Bacteroides ovatus ATCC 8483
Primary publication:
Structure and function of the DUF2233 domain in bacteria and in the human mannose 6-phosphate uncovering enzyme.
Das D, Lee WS, Grant JC, Chiu HJ, Farr CL, Vance J, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Kornfeld S, Wilson IA
J Biol Chem 288 16789-16799 (2013)
PMID: 23572527

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphodiester glycosidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 31.54 KDa
Source organism: Bacteroides ovatus ATCC 8483
Expression system: Escherichia coli
UniProt:
  • Canonical: A7LRK2 (Residues: 32-315; Coverage: 98%)
Gene name: BACOVA_00430
Sequence domains: Phosphodiester glycosidase

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand SO4 2 x SO4
Ligand EDO 17 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P61
Unit cell:
a: 96.964Å b: 96.964Å c: 91.113Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.129 0.151
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Three DUF1996 Proteins Localize in Vacuoles and Function in Fungal Responses to Multiple Stresses and Metal Ions.
Tong et al. (2016)
3 more

1 mention without citation

Structure and function of the DUF2233 domain in bacteria and in the human mannose 6-phosphate uncovering enzyme.
Das et al. (2013)