› 3ohn

X-ray diffraction
3.01Å resolution

Crystal structure of the FimD translocation domain

Released:
DOI: 10.2210/pdb3ohn/pdb
PDB entry 3ohn coloured by chain, front view.
PDB entry 3ohn coloured by chain, side view.
PDB entry 3ohn coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the FimD usher bound to its cognate FimC-FimH substrate.
Phan G, Remaut H, Wang T, Allen WJ, Pirker KF, Lebedev A, Henderson NS, Geibel S, Volkan E, Yan J, Kunze MB, Pinkner JS, Ford B, Kay CW, Li H, Hultgren SJ, Thanassi DG, Waksman G
Nature 474 49-53 (2011)
PMID: 21637253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane usher protein FimD Chains: A, B
Molecule details ›
Chains: A, B
Length: 558 amino acids
Theoretical weight: 61.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P30130 (Residues: 169-708; Coverage: 65%)
Gene names: JW5780, b4317, fimD
Sequence domains: Outer membrane usher protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 50.022Å b: 87.132Å c: 95.883Å
α: 63.92° β: 88.43° γ: 76.94°
R-values:
R R work R free
0.233 0.23 0.305
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
29 more

7 mentions without citation

The bacterial outer membrane β-barrel assembly machinery.
Kim et al. (2012)
6 more