› 3oib

X-ray diffraction
2.1Å resolution

Crystal structure of a putative ACYL-COA Dehydrogenase from mycobacterium smegmatis, Iodide soak

Released:
DOI: 10.2210/pdb3oib/pdb
Model geometry
Fit model/data
PDB entry 3oib coloured by chain, front view.
PDB entry 3oib coloured by chain, side view.
PDB entry 3oib coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Gardberg AS, Robinson JI, Christensen JS, Staker BL, Myler PJ, Stewart LJ, Edwards TE
OpenAccess logo J Struct Funct Genomics 12 83-95 (2011)
PMID: 21359836

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-106447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-CoA dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 403 amino acids
Theoretical weight: 43.18 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0R3J1 (Residues: 1-382; Coverage: 100%)
Gene name: MSMEG_5491
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand IOD 30 x IOD
Ligand EDO 2 x EDO
Ligand FDA 2 x FDA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 161.59Å b: 64.49Å c: 83.6Å
α: 90° β: 111.99° γ: 90°
R-values:
R R work R free
0.155 0.152 0.2
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Structure, mechanism, and inhibition of histone deacetylases and related metalloenzymes.
Lombardi et al. (2011)
3 more

1 mention without citation

SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth et al. (2011)