› 3ois

X-ray diffraction
1.65Å resolution

Crystal Structure Xylellain, a cysteine protease from Xylella fastidiosa

Released:
DOI: 10.2210/pdb3ois/pdb
PDB entry 3ois coloured by chain, front view.
PDB entry 3ois coloured by chain, side view.
PDB entry 3ois coloured by chain, top view.
Source organism: Xylella fastidiosa
Primary publication:
The crystal structure of the cysteine protease Xylellain from Xylella fastidiosa reveals an intriguing activation mechanism.
Leite NR, Faro AR, Dotta MA, Faim LM, Gianotti A, Silva FH, Oliva G, Thiemann OH
FEBS Lett 587 339-44 (2013)
PMID: 23333295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-192892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase C1A papain C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 32.98 KDa
Source organism: Xylella fastidiosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PGZ0 (Residues: 1-271; Coverage: 100%)
Gene name: XF_0156
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand UDP 4 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P1
Unit cell:
a: 55.089Å b: 69.314Å c: 82.368Å
α: 75.86° β: 75.43° γ: 66.51°
R-values:
R R work R free
0.168 0.166 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Radicinin from Cochliobolus sp. inhibits Xylella fastidiosa, the causal agent of Pierce's Disease of grapevine.
Aldrich et al. (2015)
2 more

2 mentions without citation

Alternative Pathway for the Reaction Catalyzed by DNA Dealkylase AlkB from Ab Initio QM/MM Calculations.
Fang et al. (2014)
1 more