› 3oj2

X-ray diffraction
2.2Å resolution

Crystal structure of FGF1 complexed with the ectodomain of FGFR2b harboring the A172F Pfeiffer syndrome mutation

Released:
DOI: 10.2210/pdb3oj2/pdb
PDB entry 3oj2 coloured by chain, front view.
PDB entry 3oj2 coloured by chain, side view.
PDB entry 3oj2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Plasticity in interactions of fibroblast growth factor 1 (FGF1) N terminus with FGF receptors underlies promiscuity of FGF1.
Beenken A, Eliseenkova AV, Ibrahimi OA, Olsen SK, Mohammadi M
J Biol Chem 287 3067-78 (2012)
PMID: 22057274

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138606 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fibroblast growth factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 1-155; Coverage: 100%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Fibroblast growth factor receptor 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 231 amino acids
Theoretical weight: 25.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21802 (Residues: 140-361, 362-368; Coverage: 28%)
Gene names: BEK, FGFR2, KGFR, KSAM
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P32
Unit cell:
a: 77.945Å b: 77.945Å c: 137.555Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.262 0.294
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Fibroblast Growth Factor signaling pathway.
Ornitz et al. (2015)
4 more

7 mentions without citation

Structural Biology of the FGF7 Subfamily.
Zinkle et al. (2019)
6 more