› 3om9

X-ray diffraction
1.98Å resolution

T. Gondii bradyzoite-specific LDH (LDH1) in complex with NAD and OXQ

Released:
DOI: 10.2210/pdb3om9/pdb
PDB entry 3om9 coloured by chain, front view.
PDB entry 3om9 coloured by chain, side view.
PDB entry 3om9 coloured by chain, top view.
Source organism: Toxoplasma gondii
Entry authors: Susko MS, Wilson DK

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 329 amino acids
Theoretical weight: 35.54 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P90613 (Residues: 3-329; Coverage: 99%)
Gene name: TGRH88_076470
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand OXQ 4 x OXQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 67.307Å b: 124.458Å c: 86.721Å
α: 90° β: 105.61° γ: 90°
R-values:
R R work R free
0.196 not available 0.223
Expression system: Escherichia coli BL21

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