› 3oml

X-ray diffraction
2.15Å resolution

Structure of full-length peroxisomal multifunctional enzyme type 2 from Drosophila melanogaster

Released:
DOI: 10.2210/pdb3oml/pdb
PDB entry 3oml coloured by chain, front view.
PDB entry 3oml coloured by chain, side view.
PDB entry 3oml coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Peroxisomal multifunctional enzyme type 2 from the fruitfly: dehydrogenase and hydratase act as separate entities, as revealed by structure and kinetics.
Haataja TJ, Koski MK, Hiltunen JK, Glumoff T
Biochem J 435 771-81 (2011)
PMID: 21320074

Function and Biology Details

Reactions catalysed: 
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194323 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal multifunctional enzyme type 2 Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 66.17 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VXJ0 (Residues: 1-598; Coverage: 100%)
Gene names: CG3415, Mfe2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 114.48Å b: 114.48Å c: 89.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.285
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Ascaroside signaling in C. elegans.
Ludewig et al. (2013)
4 more

3 mentions without citation

Active site comparisons and catalytic mechanisms of the hot dog superfamily.
Labonte et al. (2013)
2 more