› 3onh

X-ray diffraction
1.6Å resolution

Crystal structure of UBA2ufd-Ubc9: insights into E1-E2 interactions in Sumo pathways

Released:
DOI: 10.2210/pdb3onh/pdb
PDB entry 3onh coloured by chain, front view.
PDB entry 3onh coloured by chain, side view.
PDB entry 3onh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of UBA2(ufd)-Ubc9: insights into E1-E2 interactions in Sumo pathways.
Wang J, Taherbhoy AM, Hunt HW, Seyedin SN, Miller DW, Miller DJ, Huang DT, Schulman BA
PLoS One 5 e15805 (2010)
PMID: 21209884

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-activating enzyme E1-like Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52488 (Residues: 439-563; Coverage: 20%)
Gene names: D9509.10, PIP2, UAL1, UBA2, YDR390C
Structure domains: Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4
Unit cell:
a: 80.192Å b: 80.192Å c: 50.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.178
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Ubiquitin-like Protein Conjugation: Structures, Chemistry, and Mechanism.
Cappadocia et al. (2018)
6 more

1 mention without citation

Crystal structure of UBA2(ufd)-Ubc9: insights into E1-E2 interactions in Sumo pathways.
Wang et al. (2010)