› 3op2

X-ray diffraction
2Å resolution

Crystal structure of putative mandelate racemase from Bordetella bronchiseptica RB50 complexed with 2-oxoglutarate/phosphate

Released:
DOI: 10.2210/pdb3op2/pdb
PDB entry 3op2 coloured by chain, front view.
PDB entry 3op2 coloured by chain, side view.
PDB entry 3op2 coloured by chain, top view.
Source organism: Bordetella bronchiseptica
Entry authors: Malashkevich VN, Patskovsky Y, Ramagopal U, Toro R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 43.93 KDa
Source organism: Bordetella bronchiseptica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3LT39 (Residues: 2-387; Coverage: 100%)
Gene name: BB4687
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand AKG 1 x AKG
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4
Unit cell:
a: 117.55Å b: 117.55Å c: 123.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.222
Expression system: Escherichia coli BL21(DE3)

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