› 3opn

X-ray diffraction
2.05Å resolution

The crystal structure of a putative hemolysin from Lactococcus lactis

Released:
DOI: 10.2210/pdb3opn/pdb
PDB entry 3opn coloured by chain, front view.
PDB entry 3opn coloured by chain, side view.
PDB entry 3opn coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Entry authors: Zhang Z, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA methyltransferase FtsJ domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.98 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CH80 (Residues: 23-243; Coverage: 91%)
Gene names: L81441, yiiB
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 48.716Å b: 54.523Å c: 70.643Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.235
Expression system: Escherichia coli BL21(DE3)

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