› 3opy

X-ray diffraction
3.05Å resolution

Crystal structure of Pichia pastoris phosphofructokinase in the T-state

Released:
DOI: 10.2210/pdb3opy/pdb
PDB entry 3opy coloured by chain, front view.
PDB entry 3opy coloured by chain, side view.
PDB entry 3opy coloured by chain, top view.
Source organism: Komagataella pastoris DSMZ 70382
Primary publication:
Molecular architecture and structural basis of allosteric regulation of eukaryotic phosphofructokinases.
Sträter N, Marek S, Kuettner EB, Kloos M, Keim A, Brüser A, Kirchberger J, Schöneberg T
FASEB J 25 89-98 (2011)
PMID: 20833871

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108367 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP-dependent 6-phosphofructokinase subunit alpha Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 989 amino acids
Theoretical weight: 108.93 KDa
Source organism: Komagataella pastoris DSMZ 70382
UniProt:
  • Canonical: Q8NJU8 (Residues: 1-989; Coverage: 100%)
Gene name: PFK1
Sequence domains:
Structure domains:
ATP-dependent 6-phosphofructokinase subunit beta Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 941 amino acids
Theoretical weight: 103.85 KDa
Source organism: Komagataella pastoris DSMZ 70382
UniProt:
  • Canonical: Q8TGA0 (Residues: 1-941; Coverage: 100%)
Gene name: PFK2
Sequence domains: Phosphofructokinase
Structure domains:
ATP-dependent 6-phosphofructokinase subunit gamma Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 351 amino acids
Theoretical weight: 40.85 KDa
Source organism: Komagataella pastoris DSMZ 70382
UniProt:
  • Canonical: A7MAS3 (Residues: 1-351; Coverage: 100%)
Gene name: PFK3
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SO4 65 x SO4
Ligand ATP 4 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 161.658Å b: 188.303Å c: 231.56Å
α: 90° β: 92.85° γ: 90°
R-values:
R R work R free
0.247 0.2 0.232

Quick links

Citations

2 review citations

Directly from the source: endogenous preparations of molecular machines.
Mesa et al. (2013)
1 more

5 mentions without citation

Cotranslational assembly of protein complexes in eukaryotes revealed by ribosome profiling.
Shiber et al. (2018)
4 more