› 3oq8

X-ray diffraction
2.25Å resolution

Crystal structure of isocitrate lyase from Brucella melitensis, bound to the product mimic malonate

Released:
DOI: 10.2210/pdb3oq8/pdb
PDB entry 3oq8 coloured by chain, front view.
PDB entry 3oq8 coloured by chain, side view.
PDB entry 3oq8 coloured by chain, top view.
Source organism: Brucella abortus 2308
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 433 amino acids
Theoretical weight: 47.15 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YQA0 (Residues: 1-429; Coverage: 100%)
Gene name: BAB1_1631
Sequence domains: Isocitrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

3 bound ligands:
Ligand MLA 4 x MLA
Ligand EDO 11 x EDO
Ligand NA 9 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 77.5Å b: 136.03Å c: 181.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.149 0.199
Expression system: Escherichia coli

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