› 3or6

X-ray diffraction
2.7Å resolution

On the structural basis of modal gating behavior in K+channels - E71Q

Released:
DOI: 10.2210/pdb3or6/pdb
PDB entry 3or6 coloured by chain, front view.
PDB entry 3or6 coloured by chain, side view.
PDB entry 3or6 coloured by chain, top view.
Source organisms:
Primary publication:
On the structural basis of modal gating behavior in K(+) channels.
Chakrapani S, Cordero-Morales JF, Jogini V, Pan AC, Cortes DM, Roux B, Perozo E
Nat Struct Mol Biol 18 67-74 (2011)
PMID: 21186363

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209472 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody fab fragment heavy chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
antibody fab fragment light chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 10.98 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-124; Coverage: 64%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand K 7 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 155.679Å b: 155.679Å c: 76.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.268
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Modeling and simulation of ion channels.
Maffeo et al. (2012)
5 more

2 mentions without citation

On the structural basis of modal gating behavior in K(+) channels.
Chakrapani et al. (2011)
1 more